To subtract from a selection: first make initial selection and save it to Channels by pressing Add To Channels button.
![Gizmo Panel](https://gizmopanel.com/wp-content/uploads/2023/06/GizmoPanel-Luminosity-Selections-Substract.jpg)
Secondary using one of the tools: Marquee tools, the Lasso tools, the Magic Wand tool, and/or the Quick Selection tool to select an area you want to remove from the initial selection and save it to Channels by pressing Add To Channels button
![Gizmo Panel](https://gizmopanel.com/wp-content/uploads/2023/06/GizmoPanel-Luminosity-Selections-Substract2.jpg)
Click on -SEL button to load newly formed selection and marching ants around it.
![Gizmo Panel](https://gizmopanel.com/wp-content/uploads/2023/06/GizmoPanel-Luminosity-Selections-Substract3.jpg)